Abstract Detail

Poster Session

Morris, Samuel D [1], Reboredo Segovia, Ana L [1], Hughes, Karen W. [1].

Cloning of ribosomal ITS PCR products creates numerous chimeric sequences – a test involving a hybrid between Gymnopus dichrous species I and II.

Gymnopus dichrous exists in the southern Appalachians as two distinct entities with differing ITS2 sequences, G. dichrous I and II.  F₁ hybrids between the two are routinely identified but there is no evidence for F₂ or higher recombinant progeny.  Cloning of PCR products from F1 hybrids showed numerous apparent recombination events.  By comparison with sequences from single spore isolates, the putative recombinants were shown to be clone chimeras and did not reflect natural recombination processes. Chimeras and PCR-induced mutations seem to be extremely common n cloned PCR products and may result in bogus sequence data.  

1 - University of Tennessee, Ecology and Evolutionary Biology, Knoxville, TN, 37996, USA

Keywords:
Chimera
Gymnopus dichrous
Hybrid.

Presentation Type: Offered Paper - Poster
Session: P1
Location: Lincoln Room/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:00 PM
Number: P1009
Abstract ID:88
Candidate for Awards:None