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Abstract Detail

Poster Session

Lazarus, Katy [1], Smith, Matthew E. [2], Benny, Gerald L. [1].

Systematics and ecology of Syncephalis, a genus of ubiquitous mycoparasites.

Members of the genus Syncephalis (Zoopagales: Piptocephalidaceae) are ubiquitous soil fungi and obligate mycoparasites. Syncephalis species primarily attack other ‘zygomycete’ fungi but have also been recorded as parasites of Ascomycota. Although there are approximately 65 described species, DNA sequences are thus far only available for one species (Syncephalis depressa) and GenBank contains no ITS sequences from the genus. In order to generate a preliminary Syncephalis phylogeny and to facilitate the identification of Syncephalis environmental sequences from soil, we obtained isolates of Syncephalis species from across the USA. We created genus-specific primers and used them to obtain SSU, ITS, and LSU rDNA sequence data from known Syncephalis species. We also used these genus-specific primers to generate environmental sequence data directly from soil. Here we present our preliminary Syncephalis phylogeny, compare sequences from known Syncephalis species to unknown sequences in GenBank, and report on the diversity of Syncephalis detected directly in soils via environmental sequencing.

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1 - Univeristy of Florida, Plant Pathology, 1453 Fifield Hall, Gainesville, FL, 32611, USA
2 - University of Florida, Plant Pathology, 2517 Fifield Hall, Gainesville, Florida, 32611, United States

Environmental sequencing

Presentation Type: Offered Paper - Poster
Session: P7
Location: Lincoln Room/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:00 PM
Number: P7009
Abstract ID:69
Candidate for Awards:Graduate Student Poster Presentation Award

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