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Abstract Detail


Adenipekun, Dr.Clementina Oyinkansola [1], Ipeaiyeda, Dr Ayodele R [2], Egbewale , Samson O. [3].

Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) in spent and fresh cutting fluids polluted soils by a white-rot fungus Pleurotus ostreatus (Jacq.)Fr.Kumm.

In this study, the ability of  Pleurotus ostreatus in the degradation of Polycyclic Aromatic Hydrocarbons (PAHs) in soils contaminated with spent(SCF) and fresh cutting fluids (FCF) was investigated.A known weight of soil was contaminated with varying concentrations(10, 20,30 %) of  SCF and FCF was inoculated with P. ostreatus, incubated at 28 ± 2 â°C for 2 months and analyzed in triplicates for 16  priority PAHs.The samples were analyzed using AP gas chromatogram model 6890 powered with HP chemstation Rev A. 09.01 (1206) software. Before inoculation (0 month), PAHs in the cutting fluids were mainly composed of 2 to 6 fused benzene rings. After 2 months of incubation, there were significant reductions in concentrations of PAHs in both SCF and FCF soils. The remaining total PAHs in  SCF soil  were 56.98%, 58.15% , 65.89%   and  1.56%, 1.32%, 51.01%  for FCF soil at 10 , 20  and 30 % respectively .The overall PAHs degradation of 98.44 %, 98.68%,48.99 % were obtained for FCF soils and 43.02 %, 41.85 % and 34.11 %  for 10,20 and 30% SCF soils respectively. Also, among the individual PAHs, Benzo (a) pyrene in FCF soil contaminated soil had the highest PAHs reduction of 99.77% and 99.78% at 10 and 20% contamination. Conversely, highest PAHs reduction was observed in Benzo (g,h,i) perylene of SCF contaminated soil with 90.51%, 94.13% ,74.05% at 10, 20 and 30% concentrations .Thus, P. ostreatus is suitable as a potent agent for biodegradation of PAHs in contaminated soils. ACKNOWLEDGEMENTS. The authors wish acknowledge  the support of the University of Ibadan, Ibadan, Nigeria  Senate Research Grant SRG/FS/2010/19A for funding this Research.

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1 - University of Ibadan, Department of Botany, Ibadan, Oyo, 234, Nigeria
2 - University of Ibadan, Chemistry, Ibadan, Oyo, 234, Nigeria
3 - University of Ibadan, Botany, Ibadan, Oyo, 234, Nigeria

white-rot fungus
polluted soils.

Presentation Type: Offered Paper - Paper
Session: 1
Location: Room 103 AB/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:45 AM
Number: 1002
Abstract ID:63
Candidate for Awards:None

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