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Abstract Detail



Genetics/Molecular Biology

Diaz Valderrama, Jorge Ronny [1], Aime, M. Catherine [1].

Moniliophthora roreri (Marasmiaceae) possesses a tetrapolar mating system but appears to reproduce clonally.

Moniliophthora roreri, causal agent of frosty pod rot of cacao, belongs to the Marasmiaceae (Agaricales) but no mushroom fruiting body has ever been observed for this fungus, which is only known to reproduce by the production of conidia. We examined the evidence for potential cryptic sexual reproduction via two methods. First, we produced a draft genome for M. roreri in order to identify potential A (homeodomain transcription factors HD1 and HD2) and B (pheromone precursors and pheromone receptors) mating genes. Specific primers were then designed to screen mating types across a set of 48 geographically distinct isolates. Secondly, an 11-marker microsatellite set was developed and screened across the same pool of isolates, followed by a principal component analysis (PCA). We found evidence that M. roreri possesses a tetrapolar mating system, as is true for many other investigated Marasmiaceae. However, mating allele frequency distributions and PCA support a model of clonality in M. roreri.


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1 - Purdue University, Botany and Plant Pathology, 915 West State Street, West Lafayette, IN, 47907, USA

Keywords:
heterothallism
anamorphic
cacao diseases
compatibility
Theobroma
tropical plant pathogen.

Presentation Type: Offered Paper - Paper
Session: 3
Location: Room 104 AB/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 9:00 AM
Number: 3003
Abstract ID:60
Candidate for Awards:Graduate Student Oral Presentation Award


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