| Abstract Detail
Lignocellulose Deconstruction by Fungi Xiong, Yi [1], Glass, Louise [2]. Exploring plant cell wall degradation using Neurospora crassa. Neurospora crassa is an early colonizer of burnt vegetation in the wild and grows robustly on polysaccharides that are present in plant cell walls. We have employed N. crassa as a model filamentous fungus to understand how saprobic  fungi sense the presence of plant biomass and adjust their transcriptome, proteome, and phosphoproteome to utilize different carborn sources from plant cell walls. By screening the N. crassa near-full genome deletion strain set we have identified transcription factors (TFs) that are essential for cellulose and hemicellulose utilization, as well as mutants with increased or decreased secretion of cellulolytic enzymes. We have further characterized the functions of these TFs through analyses of the mutants by RNA-seq and by mis-expression of the TFs. We have also examined changes in the global proteomic and phosphoproteomic profiles when N. crassa is transferred from sucrose media to carbon-starvation or cellulose conditions. A comparison between the proteomes and the transcriptomes under identical growth conditions indicates extensive post-transcriptional regulations that occur  in response to exposure to cellulosic material.
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1 - UC berkeley, Energy Biosciences Institute, 2151 Berkeley Way, Berkeley, CA, 94720, USA
2 - UC berkeley, Energy Biosciences Institute, Plant and Microbiology, 341A Koshland Hall, Berkeley, CA, 94720, United States
Keywords:
Neurospora crassa
cellulase
hemicellulase
transcriptome
proteome
phosphoproteome.
Presentation Type: Symposium or Colloquium Presentation
Session: SY9
Location: Room 104 AB/Kellogg Hotel and Conference Center
Date: Wednesday, June 11th, 2014
Time: 3:00 PM
Number: SY9005
Abstract ID:59
Candidate for Awards:None |
