Abstract Detail

Poster Session

Bourret, Tyler [1], Sitepu, Irnayuli [2], Schiff, Nathan [3], Ignatia, Laura [4], Kanti, Atit [5], Faulina, Sarah [6], Silaban, Sira [6], Nugroho, Agustinus [5], Chow, Elaine [2], Nguyen, Ngoc-Lien [2], Ubaidillah, Rosichon [5], Rizzo, David [1], Boundy-Mills, Kyria [2].

Diversity of yeasts and filamentous fungi associated with wood-feeding beetles in Indonesia.

Fungi were isolated from wood-feeding insects, primarily beetles, and their galleries in infested wood at two tropical rainforest sites on the island of Sulawesi, Indonesia. Ecological associations between culturable fungal communities and beetle families were analyzed. Beetle larvae were identified using mtCOI sequences. Larvae were surface sterilized, dissected, and their gut contents were plated on Rose Bengal Chloramphenicol Agar. After isolation, more than 250 strains of yeasts were identified using sequences of the D1/D2 region of the large subunit rDNA, and more than 350 strains of filamentous fungi using ITS rDNA. More than 200 fungal species were identified, including members of subphyla Agaricomycotina, Mortierellomycotina, Mucoromycotina, Pezizomycotina, Pucciniomycotina and Saccharomycotina. The collection includes many taxa that have not had barcode sequences deposited in GenBank, and may not have been previously described. The difficulty of assigning strains to described species was due to a combination of lack of proper resolution by barcoding loci, ambiguous species boundaries, lack of voucher sequences in GenBank and taxonomic novelty of isolates. Yeasts and filamentous fungi were largely restricted to separate subphyla; within Agaricomycotina, yeasts were restricted to Tremellomycetes, while filamentous fungi were Agaricomycetes. Most yeasts isolated were Saccharomycotina, with the xylose-fermenting, insect-associated genus Scheffersomyces well represented. The filamentous fungi were represented by many genera known as wood decayers, as plant pathogens, and as ubiquitous saprotrophs such as Aspergillus, Mucor and Penicillium; Trichoderma harzianum Rifai was the most commonly isolated species. More than half of the filamentous isolates were members of Sordariomycetes, a highly diverse class of ascomycete fungi containing the majority of described, insect-vectored fungal plant pathogens. Numerous strains of Fusarium and other Hypocreales were isolated, but none belonging to Ophiostomatales. Many novel associations between fungi and beetles were identified in the study which may aid quarantine efforts aimed at reducing transmission of beetle-vectored plant pathogens. Microbes have been deposited in three microbial culture collections: the Forest Microbe Collection (FORDACC), Forest Research and Development Agency, Ministry of Forestry, Bogor, Indonesia; the Indonesian Microbe Collection (InaCC), Research Center for Biology, Cibinong, Indonesia; and the Phaff Yeast Culture Collection (UCDFST), University of California Davis. The collection is a living profile of the fungal communities of common but often overlooked substrata, and represents a valuable tool for a variety of research uses.

1 - University of California, Davis, Plant Pathology, Department of Plant Pathology, One Shields Ave, Davis, CA, 95616-8751, USA
2 - University of California, Davis, Food Science & Technology
3 - USDA Forest Service, Southern Research Center, Stoneville, MS, USA
4 - University of California, Davis, Department of Plant Biology
5 - Institute of Sciences (LIPI), Biology division, Cibinong, Indonesia
6 - Ministry of Forestry, Forestry Research and Development Center, Bogor, Indonesia

Keywords:
yeast
mold
beetle
Sulawesi
Indonesia
fungal ecology
vector.

Presentation Type: Offered Paper - Poster
Session: P7
Location: Lincoln Room/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:00 PM
Number: P7004
Abstract ID:38
Candidate for Awards:Graduate Student Poster Presentation Award