| Abstract Detail
Genetics/Molecular Biology Gilbert, Matthew [1], Mack, Brian [1], Bhatnagar, Deepak [1], Cary, Jeff [1]. RNA-Seq Analysis of an nsdC Mutant in Aspergillus flavus. The C2H2-type transcription factor NsdC (Never in Sexual Development C) has been shown to play a role in asexual development and secondary metabolite production in Aspergillus flavus, an agriculturally relevant, aflatoxin-producing species. The nsdC knoackout mutant demonstrates perturbed morphological development of conidiophores, altered pigmentation and loss of sclerotia and aflatoxin production. To further our studies on the role of nsdC in development and aflatoxin production we conducted RNA-Seq analysis of an A. flavus nsdC knockout mutant (Af70 S-strain parent) at two distinct developmental time points. At six hours post-plating the nsdC mutant showed premature conidiation and altered conidiophore morphology compared to a pyrG transformed control line, and at 54 hours a lack of aflatoxin and sclerotial production. RNA-Seq data collected at these developmental stages revealed disruption to metabolic and other developmentally-related pathways. Gene enrichment analysis, pathway analysis, and functional data will be presented to further elucidate the biological role of nsdC.
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1 - USDA, Food and Feed Safety, 1100 Robert E Lee, New Orleans, LA, 70124, United States
Keywords:
Aspergillus
RNA-seq.
Presentation Type: Offered Paper - Paper
Session: 3
Location: Room 104 AB/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 9:30 AM
Number: 3005
Abstract ID:215
Candidate for Awards:None |
