Abstract Detail

Cell Biology/Physiology

Ramírez-Del Villar, Arianne [1], Callejas-Negrete, Olga A. [1], Riquelme, Meritxell [1], Mouriño-Pérez, Rosa R. [1].

The class V myosin in Neurospora crassa.

Filamentous fungi grow in polarized fashion, though the secretion of vesicles to the cell apex. Vesicle movement is produced by motor protein via the cytoskeleton. For actin the group of motor proteins are called myosins. Class V myosin is responsible for intracellular vesicle transport in diffrent organisms. We studied the dynamics and localization of the only class V myosin in Neurospora crassa tagged with GFP and characterize a Myo-5 deletion mutant. MYO-5-GFP is localized in the tip, partially co-locating with Spitzenköper forming to dome. The photobleaching studies showed that MYO-5-GFP is transported from the subapical region toward to the tip. The absence of class V myosin produces alterations in growth and morphology of the hyphae. Colonies of  Myo-5 mutant are smaller and more compact than the WT strain. Myo-5 mutant produced 22.8 % of the WT biomass. Observations by confocal microscopy show differences in cell wall distribution in conidia, germlings and mature hyphae between the Myo-5 mutant and the WT. Moreover, in mutant hyphae the Spitzenkörper is clearly disorganized, observed as a diffuse cloud. Conidia in the Myo-5 mutant are bigger (2 X) and very rare. In conclusion Myo-V in N. crassa is not essential, but plays an important role in morphogenesis and growth in N. crassa.

1 - Centro de Investigación Cientifica y de Estudios Superiores de Ensenad, Microbiology, Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada, BC, 22860, México

Keywords:
 class V myosin
Vesicle movement.

Presentation Type: Offered Paper - Paper
Session: 6
Location: Room 104 AB/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 1:45 PM
Number: 6004
Abstract ID:187
Candidate for Awards:Graduate Student Poster Presentation Award