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Abstract Detail

Poster Session

Villavicencio-Aguilar, Fausto M. [1], Callejas-Negrete, Olga A. [1], Mouriño-Pérez, Rosa R. [1].

AMI-1 a microtubule +TIP Associated to nuclear displacement in Neurospora crassa .

Microtubule organization depends on several associated proteins (MAPs), these proteins are related to the plus or minus end of cytoplasmic or mitotic microtubules (MTs). In filamentous fungi, the behavior of astral MTs and the movement of the mitotic spindle seem to be regulated by a plus end protein ApsA (anucleate primary sterigmata), first described in Aspergillus nidulans. We found the homolog of ApsA in Neurospora crassa and we named it AMI-1. The N. crassa ami-1 gen has a high identity with ApsA and AMI1 of Podospora anserina. In this study we analyzed the effect of the lack of AMI-1 in a deletion mutant and observed the nuclear dynamics and the kinetic features of the null mutant during vegetative growth, asexual and sexual reproduction in comparison with a wild type strain (WT). We are showing preliminary data about the cellular features of the Δami-1 deletion mutant compared with the WT. The Δami-1 mutant showed a decrease of the longation rate of 34%. Although hyphal growth is polarized, hyphae in the mutant grow in a meandering fashion. The mutant displays a lower number of branches and is hyperseptated. The cell wall distribution in the Δami-1 mutant is abnormal; there is a higher concentration close to the hyphal tips. So far, we can conclude that the ami-1 gene of N. crassa is not essential but affects cell growth probably due the effect of the alterations in nuclear distribution.

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1 - Centro de Investigación Cientifica y de Estudios Superiores de Ensenad, Microbiology, Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada, BC, 22860, México

 microtubule +TIP
nuclear displacement.

Presentation Type: Offered Paper - Poster
Session: P1
Location: Lincoln Room/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:00 PM
Number: P1001
Abstract ID:179
Candidate for Awards:Graduate Student Poster Presentation Award

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