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Abstract Detail

Genetics/Molecular Biology

Hallen-Adams, Heather [1], Pribil, Erica [1].

Inter- and intraspecific diversity of Candida colonizing the healthy human gastrointestinal tract.

Candida yeasts are considered dominant members of the human commensal fungal flora, or mycobiome. It is common to observe a single species or strain dominating a niche (skin, oropharyngeal cavity, ileum, colon, vagina, etc.), multiple Candida species, or multiple strains of a single species may coexist. Amplicon pyrosequencing of fungal ITS DNA from feces collected from 44 healthy adult volunteers produced six samples containing three Candida yeasts each: the commensals and opportunistic pathogens C. albicans and C. tropicalis, and Candida sake of presumed environmental origin. ITS sequence variation within species, both within and between samples (different participants and/or different timepoints) are discussed. Notably, the commensal Candida yeasts exhibit remarkably high sequence divergence, both genome-wide and within the ITS region, and an argument can be made both for multiple colonizations with different strains, and for mutation and sequence divergence following colonization.

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1 - University of Nebraska-Lincoln, Food Science and Technology, 143 Filley Hall, Lincoln, NE, 68583-0919, USA

Candida albicans
Candida tropicalis
Candida sake
DNA sequence analysis.

Presentation Type: Offered Paper - Paper
Session: 4
Location: Auditorium/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 2:00 PM
Number: 4005
Abstract ID:143
Candidate for Awards:None

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