Create your own conference schedule! Click here for full instructions

Abstract Detail



Genetics/Molecular Biology

Hallen-Adams, Heather [1], Pribil, Erica [1].

Inter- and intraspecific diversity of Candida colonizing the healthy human gastrointestinal tract.

Candida yeasts are considered dominant members of the human commensal fungal flora, or mycobiome. It is common to observe a single species or strain dominating a niche (skin, oropharyngeal cavity, ileum, colon, vagina, etc.), multiple Candida species, or multiple strains of a single species may coexist. Amplicon pyrosequencing of fungal ITS DNA from feces collected from 44 healthy adult volunteers produced six samples containing three Candida yeasts each: the commensals and opportunistic pathogens C. albicans and C. tropicalis, and Candida sake of presumed environmental origin. ITS sequence variation within species, both within and between samples (different participants and/or different timepoints) are discussed. Notably, the commensal Candida yeasts exhibit remarkably high sequence divergence, both genome-wide and within the ITS region, and an argument can be made both for multiple colonizations with different strains, and for mutation and sequence divergence following colonization.


Log in to add this item to your schedule

1 - University of Nebraska-Lincoln, Food Science and Technology, 143 Filley Hall, Lincoln, NE, 68583-0919, USA

Keywords:
microbiome
Candida albicans
Candida tropicalis
Candida sake
DNA sequence analysis.

Presentation Type: Offered Paper - Paper
Session: 4
Location: Auditorium/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 2:00 PM
Number: 4005
Abstract ID:143
Candidate for Awards:None


Copyright 2000-2013, Botanical Society of America. All rights reserved