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Abstract Detail



Poster Session

Finlayson, Timothy John [1].

Fungal-mediated biotransformation of DHEA (didehydroepiandrosterone) by Penicillium coprobium is associated with increased antimicrobial activity against bacteria and fungi.

Timothy J. Finlayson, finlaysont@findlay.edu, Kara Bloom, bloomk@findlay.edu, Rahul Khupse, khupse@findlay.edu, Michael A. Edelbrock, edelbrock@findlay.edu, Donald M. Walker, walkerd@findlay.edu, The University of Findlay, Dept of Natural Sciences, Findlay OH 45840.    
Fungi are important in the production of medicine accounting for more than 10-20% of the most profitable human drugs. These medicines are critical for the maintenance and improvement of human health.  Fungi are extraordinary metabolite and novel compound producers. They form chemically and structurally complex compounds many of which possess antimicrobial activity. Fungal-mediated biotransformation has the advantage of increased regio- and stereo-selectivities using environmentally friendly reaction conditions. During this experiment, the metabolic pathway of Penicillium coprobium was directed to biotransform DHEA (dehydroepiandrosterone), which is hormone manufactured naturally by the body's adrenal glands, and is commonly available as an over the counter dietary supplement. Penicillium coprobium was grown in two different types of liquid culture, potato dextrose broth (PDB) and PDB with a DHEA additive. A solid-liquid and solvent (ethyl acetate) partitioning protocol was used to extract the total organic compounds produced. The raw extracts (PDB and PBD+DHEA) were screened under standardized conditions using the Kirby-Bauer disc diffusion assay for antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae. Penicillium coprobium showed a significant increase in antimicrobial activity against E. coli (F1, 82 = 6.2; p = 0.0001), S. aureus (F1, 86 = 6.0; p = 0.0001), and S. cerevisiae (F1, 123 = 4.36; p = 0.0001) in the biotransformed PBD+DHEA extract when compared to the PDB extract. Thin layer chromatography (UV light and KMnO4 stain) was used to support that P. coprobium metabolizes DHEA resulting in different metabolites. The biotransformed compounds produced in this experiment by P. coprobium may be potential leads for novel antimicrobial drugs.


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1 - The University of Findlay, Pharmacy, 602 Howard Street, Findlay, OH, 45840, USA

Keywords:
Biotransformation by P. coprobium.

Presentation Type: Offered Paper - Poster
Session: P1
Location: Lincoln Room/Kellogg Hotel and Conference Center
Date: Monday, June 9th, 2014
Time: 8:00 PM
Number: P1003
Abstract ID:104
Candidate for Awards:None


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