Abstract Detail
Evolution of Hyphal Function and Development Schultzhaus, Zachary [1]. Membrane Trafficking and Hyphal Tip Growth in Aspergillus nidulans. Hyphae of filamentous fungi grow in a characteristic, polarized manner involving a unique set of membrane trafficking behaviors including tip-localized exocytosis and highly concentrated, subapical endocytosis. Endocytic recycling, a process which returns internalized proteins and lipids to the plasma membrane, has been proposed as a mechanism that may keep some polarity markers at hyphal tips to enable rapid growth. Indeed, membrane recycling is known to maintain polarized localization of phospholipid translocases and vesicle targeting proteins in budding yeast. In this study, we recorded internal membrane flow in the hyphal tips of Aspergillus nidulans using clathrin heavy chain-GFP as a reporter, and observed several structures that reveal the fragile and dynamic nature of hyphal tips. We also examined the effects of blocking the interface between the endocytic and secretory pathways, and determined that membrane recycling is crucial for the maintenance of hyphal shape and rapid polarized growth.
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1 - Texas A&M University, Department of Plant Pathology and Microbiology, 2132 TAMU, LF Peterson Building, College Station, TX, 77845, USA
Keywords:
Cell Polarity
Hyphal Growth.
Presentation Type: Symposium or Colloquium Presentation
Session: SY6
Location: Room 104 AB/Kellogg Hotel and Conference Center
Date: Tuesday, June 10th, 2014
Time: 3:00 PM
Number: SY6005
Abstract ID:24
Candidate for Awards:None | 